Natural Vanillin Production from Isoeugenol by Using Pseudomonas putida in Biphasic Bioconversion Medium

Vanillin, which is principal aroma constituent of the beans obtained from vanilla orchids, one most demanded flavoring agents in world. Because insufficient supply natural vanillin, market demand usually supplied by synthetic ones. Taking into consideration consumer trends, interest to products are rising day instead counterparts. In this study, it was investigated possibility usage biphasic system bioconversion process isoeugenol vanillin Pseudomonas putida (HUT 8100) culture. Organic phase composed as sole carbon source medium while biocatalyst, P. culture, dispersed inorganic phase. Incubation performed at 28 ○C, pH 6.3 and 180 rpm shaking. Isoeugenol amounts were simultaneously analyzed HPLC 270 nm. has low solubility water tested concentrations ranged between 5-60% (w/w) compose system. various initial substrate concentrations, reached highest level containing 40% after 120 h. specified conditions, consumption production following periodically for 17 days. According results, determined that 11.95 g L-1 produced with 6.2% molar yield end 15 days bioconversion. It thought that, result using very important industrial applications via